The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice Nike Air Max 1 Canada
without off-target mutations.
Parallel algorithms are given for finding a maximum weighted clique, Air Max 95
a maximum weighted independent set, a minimum clique cover, and a minimum weighted dominating set of an interval graph. Parallel algorithms are also given for finding a Hamiltonian circuit and the minimum bandwidth of a proper interval graph. The shared memory model (SMM) of parallel computers is used to obtain fast algorithms.
The University of British Columbia (UBC) Education Library used information about library opening hours world-wide and the results of an in-house 4-week survey of students exiting the Library between 9 and 10 p.m. to decide on new hours of operation that would better meet the needs of graduate students and eliminate confusion over changing seasonal hours.
The existence of an overfull subgraph of a simple graph implies χ′ Δ+1, where χ′ denotes the chromatic index and Δ the maximum degree. A fast algorithm for finding overfull subgraphs of simple graphs with 2Δ⩾|V|is presented.